anti cd8  (Bioss)

Journal: Biomolecules

Article Title: Human Mutant Dynactin Subunit 1 Causes Profound Motor Neuron Disease Consistent with Possible Mechanisms Involving Axonopathy, Mitochondriopathy, Protein Nitration, and T-Cell-Mediated Cytolysis

doi: 10.3390/biom15121637

Figure Lengend Snippet: T cells invade the spinal cord parenchyma of mutant-DCTN1 tg mice. ( A ) Localization of Fas-positive cells (thin arrows) in ventral horn of wildtype DCTN1 transgenic mouse. Scale bar (same for ( B )) = 40 µm. ( B ) Fas-positive cells (thin arrows) in the ventral horn of mutant DCTN1 tg mice appear more numerous than in wildtype tg mice. Inset is dashed square showing Fas-positive cells in the vicinity of a motor neuron. Inset scale bar = 12 µm. ( C ) Fas-positive cells (thin arrows) near degenerating motor neurons (asterisk) in mutant-DCTN1 mice. Scale bar = 7 µm. ( D ) Box plot showing the mean Fas-positive cell number (with IQR and 5–95 percentile whiskers, n = 6/group) in the ventral horn of age-matched non-tg, human wildtype DCTN1 (p150) and human mutant DCTN1 (p150) at 8 months of age. ( E ) Infiltrated CD8-positive cells (arrows) in the ventral horn of mutant DCTN1 mice. CD8 immunoreactivity is also present in the surrounding white matter (wm). Scale bar = 12 µm. ( F ) Focal accumulations of CD8 immunoreactivity were seen in the ventral root exit zones in the ventral funiculus of mutant DCTN1 mice. Scale bar = 15 µm.

Article Snippet: The sections were blocked and permeabilized (1 h) in 10% normal goat serum (NGS) with 0.4% Triton-x 100 and then incubated (4 °C) in primary IgG antibodies overnight: rabbit monoclonal anti-cleaved caspase-8 (1:500, D5B2), rabbit monoclonal anti-cleaved caspase-3 (1:4000, D3E9), rabbit polyclonal anti-SOD2 (1:2000), rabbit polyclonal anti-Parkin (1:500), rabbit polyclonal anti-mouse TNF-α (Chemicon, St. Louis, MO, USA, 1:500), rabbit monoclonal anti-IL9 (Abcam, Cambridge, MA, USA, 1:500), monoclonal anti-nitrated Hsp90 (1:1000), a mouse monoclonal anti-Fas (BD Transduction Laboratories, Franklin Lakes, NJ, USA, 1:500), and a rabbit polyclonal antibody to CD8 (Proteintech, 1:500).

Techniques: Mutagenesis, Transgenic Assay

Journal: Bioactive Materials

Article Title: Light-cured millineedle platform delivers “nano-pomegranate” for combinatorial electrodynamic therapy and cuproptosis against oral carcinoma

doi: 10.1016/j.bioactmat.2025.07.032

Figure Lengend Snippet: In vivo antitumor efficiency of N-PG/NSC + Cu 2+ MNs combined with R848-based immunotherapy (n = 4). (A) Schematic diagram of the treatments. Volume growth curves of the tumors on the back (B) and neck (C) during treatments. Photographs of the back tumors (D) at the end of treatments ex vivo and (E) during the treatments in situ . Photographs of the neck tumors ex vivo (F) and in situ (G) at the end of treatments. (H) Representative flow cytometry plots and (I–J) the corresponding quantitative analysis of CD4 + and CD8 + T cells (gated on CD3 + T cells) in tumor tissues at the end of various treatments. (K) Representative flow cytometry plots and (L) the corresponding quantitative analysis of Foxp3 + Treg cells (gated on CD3 + CD4 + T cells) in tumor tissues at the end of various treatments. Data are shown as the mean values ± SD (n = 4). ∗∗P < 0.01, ∗P < 0.05, comparison between two treatment groups.

Article Snippet: Primary monoclonal antibodies against Cyt c (Cat: bs-0013R), DLAT (Cat: bs-19695R), LIAS (Cat: bs-1824R), HSP70 (Cat: bs-0126R), Ki67 (Cat: bs-2130R), CD4 (Cat: bs-0647R), CD8 (Cat: bs-10699R) and Alexa Flour 488-labeled monoclonal antibody against calreticulin (Cat: bs-5913R-AF488) were obtained from Bioss Biotechnology (Beijing, China).

Techniques: In Vivo, Ex Vivo, In Situ, Flow Cytometry, Comparison